منابع مشابه
Duplex DNA
This article describes the sequence-specific isolation and purification of intact double-stranded DNA (dsDNA) by oligonucleotide/PNA-assisted affinity capture (OPAC). The OPAC assay is based on selective tagging of a DNA duplex by biotinylated oligodeoxyribonucleotide (ODN) through formation of a so-called PD-loop. The PD-loop is assembled with the aid of a pair of PNA “openers”, which allow se...
متن کاملPNA beacons for duplex DNA.
We report here on the hybridization of peptide nucleic acid (PNA)-based molecular beacons (MB) directly to duplex DNA sites locally exposed by PNA openers. Two stemless PNA beacons were tested, both featuring the same recognition sequence and fluorophore-quencher pair (Fluorescein and DABCYL, respectively) but differing in arrangement of these groups and net electrostatic charge. It was found t...
متن کاملFlavopiridol binds to duplex DNA.
Flavopiridol, the first potent cyclin-dependent kinase inhibitor to enter clinical trials, was recently found to be cytotoxic to noncycling cells. The present studies were performed to examine the hypothesis that flavopiridol, like several other antineoplastic agents that kill noncycling cells, might also interact with DNA. Consistent with this possibility, treatment of A549 human lung cancer c...
متن کاملThe H2O Helix: The Chiral Water Superstructure Surrounding DNA
The double helix structure of DNA comes as close as it gets in science to a household brand. Results reported by Petersen and co-workers uncover a new structure buried just at the surface of DNA: a chiral, helical superstructure of water molecules (Figure 1). The authors applied second-order nonlinear optical spectroscopy to observe the collective orientation of these waters. They propose that ...
متن کاملHelix-coil transition of parallel-stranded DNA. Thermodynamics of hairpin and linear duplex oligonucleotides.
The stabilities have been determined of different DNA double helices constructed with the two constituent strands in a parallel orientation. These molecules incorporate polarity-inverting loop structures (hairpins) or nucleotide sequences (duplexes) which impose the desired polarity on the two strands constituting the sugar-phosphate backbone. The hairpins consisted of d(A.T)n stems (n = 8 or 1...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1976
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.73.11.4125